Improving non-viral knock-in with modified single-stranded DNAs and small molecules
Nature Biotechnology, Published online: 25 August 2022; doi:10.1038/s41587-022-01420-0The addition of Cas9 target sequences to long single-stranded DNAs, combined with cocktails of small molecules to boost homology-directed repair, leads to marked enhancement of non-viral knock-in efficiency and yield in primary human T cells and other hematopoietic cell types…
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